The pre-print report , now awaiting review from scientific peers prior to publication, showed that deer may have picked up the virus from humans sometime during the testing period, between April and January , though Penn State researchers are not clear as to how cross-transmission could have occurred.
Their samples were derived from both roadkill and deer felled by hunters. An analysis of their lymph nodes reflected a genomic sequencing that hinted the virus had first traveled through humans before infecting the deer. Researchers and Iowa wildlife officials are sounding the alarm, particularly for deer hunters and other animal handlers, warning them to take extreme precautions with the animal in nature.
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Virologist and associate professor at the University of Queensland Ian Mackay showed in this blog post how multiple scientific studies demonstrated that SARS-CoV-2 was isolated independently in multiple labs across the world. These studies, which are freely accessible to the public on the Internet, provide evidence that the virus does indeed exist and furthermore, is not being confused with other viruses.
The study was led by researchers at the Wuhan Institute of Virology and published in Nature. Figure 1. Modified from Extended Data Figure 6, Zhou et al. Mackay described their study thus:. They took this virus and centrifuged all the uninfected cells and cell debris and made stocks into a pellet, and collected and froze the liquid above the inactivate the supernatant.
Some of this stock was later thawed and used to infect a fresh new flask of Vero E6 cells. After six hours the cultures were fixed treated with a chemical to kill the cells and inactivate the virus while retaining the cell and virus structures—like freezing them in time. They now had a very pure preparation. There are no Vero E6 cells or bacteria or broken up pieces left, just the tiny virus particles.
This was then prepared for cryo-electron tomography studies which allowed the investigators to view and produce images of single virions and even individual projecting spike protein trimers.
Some amazing detail can be seen below.
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